sali restriction enzymes (New England Biolabs)
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sali restriction enzymes
Sali Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sali+restriction+enzyme/Sal+I/bio_rxiv__64898__2026__05__06__723300-162-12-15
Average 97 stars, based on 3296 article reviews
Sali Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sali+restriction+enzyme/Sal+I/bio_rxiv__64898__2026__05__06__723300-162-12-15
Average 97 stars, based on 3296 article reviews
sali restriction enzymes - by Bioz Stars,
2026-09
97/100 stars
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Amplification:Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome. Article Snippet: Three independent clones carrying an integrated form of pOJ260-SAMYPH94 and three independent stocks of the S. ambofaciens ATCC 23877 strain were grown for three days in TSB medium before being collected for phenol extraction of gDNA. .. To prevent amplification bias due to DNA topology (Hou et al. 2010), both gDNAs and the pOJ260_SAMYPH94 plasmid were linearized using the Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome Article Snippet: Three independent clones carrying an integrated form of pOJ260- SAMYPH94 and three independent stocks of the S. ambofaciens ATCC 23877 strain were grown for three days in TSB medium before being collected for phenol extraction of gDNA. .. To prevent amplification bias due to DNA topology (Hou et al. ), both gDNAs and the pOJ260_ SAMYPH94 plasmid were linearized using the Plasmid Preparation:Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome. Article Snippet: Three independent clones carrying an integrated form of pOJ260-SAMYPH94 and three independent stocks of the S. ambofaciens ATCC 23877 strain were grown for three days in TSB medium before being collected for phenol extraction of gDNA. .. To prevent amplification bias due to DNA topology (Hou et al. 2010), both gDNAs and the pOJ260_SAMYPH94 plasmid were linearized using the Article Title: Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana. Article Snippet: .. To generate the dysfunctional AcMYB110 construct, the AcMYB110 sequence of the AcMYB110 overexpression construct (pCAMBIA- 35S::AcMYB110) was subjected to digestion with Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization. Article Snippet: 2.2 Plasmid Amplification and Transformation of P. pastoris The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization Article Snippet: The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Article Title: A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome Article Snippet: Three independent clones carrying an integrated form of pOJ260- SAMYPH94 and three independent stocks of the S. ambofaciens ATCC 23877 strain were grown for three days in TSB medium before being collected for phenol extraction of gDNA. .. To prevent amplification bias due to DNA topology (Hou et al. ), both gDNAs and the pOJ260_ SAMYPH94 plasmid were linearized using the Article Title: Recombinant flounder Egf increases southern flounder sperm motility and fertility at the end of the reproductive season Article Snippet: .. Prior to yeast transformation, the pPIC9K vector containing the synthetic gene was linearized with Construct:Article Title: Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana. Article Snippet: .. To generate the dysfunctional AcMYB110 construct, the AcMYB110 sequence of the AcMYB110 overexpression construct (pCAMBIA- 35S::AcMYB110) was subjected to digestion with Sequencing:Article Title: Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana. Article Snippet: .. To generate the dysfunctional AcMYB110 construct, the AcMYB110 sequence of the AcMYB110 overexpression construct (pCAMBIA- 35S::AcMYB110) was subjected to digestion with Over Expression:Article Title: Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana. Article Snippet: .. To generate the dysfunctional AcMYB110 construct, the AcMYB110 sequence of the AcMYB110 overexpression construct (pCAMBIA- 35S::AcMYB110) was subjected to digestion with Recombinant:Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization. Article Snippet: 2.2 Plasmid Amplification and Transformation of P. pastoris The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization Article Snippet: The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Purification:Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization. Article Snippet: 2.2 Plasmid Amplification and Transformation of P. pastoris The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization Article Snippet: The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Spectrophotometry:Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization. Article Snippet: 2.2 Plasmid Amplification and Transformation of P. pastoris The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Article Title: Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization Article Snippet: The recombinant plasmid rHA1-pPIC9K was propagated in E. coli DH5α cells and amplified by cultivation in Luria-Bertani (LB) medium supplemented with ampicillin at 37 °C for 12 h. Plasmid DNA was isolated using a commercial miniprep kit (Sangon Biotech, China). .. For genomic integration, the recombinant plasmid was linearized with Transformation Assay:Article Title: Recombinant flounder Egf increases southern flounder sperm motility and fertility at the end of the reproductive season Article Snippet: .. Prior to yeast transformation, the pPIC9K vector containing the synthetic gene was linearized with |